Laser Ablation Based Fast Prototyping of Fluidic Diode and Finger-driven Microdevice for Precise Metering and Delivery of Multi-source Liquid Reagents

In this work, we report a novel method of fabricating Glass-PDMS-glass fluidic diode using CO2 laser ablation that avoids conventional laborious procedures such as spin coating and molding of PDMS. By using this type of fluidic diode we constructed a finger-driven microdevice that can meter and deliver (MAD) multiple solutions in parallel, and the efficiency and accuracy of the MAD function was proved to be comparable with conventional methods. Polymerase chain reaction (PCR) mixture can be prepared using this finger-driven microdevice without inhibiting the amplification. The prototype of the microdevice provides a potential platform for point-of-care applications